Measurement of Circular Dichroism Dynamics in a Temperature Jump Experiment for the Study of Protein Folding
نویسنده
چکیده
The question how proteins fold into their specific three-dimentional structures provides anexciting challenge for biophysicists nowadays. The use of a fast temperature-jump is a verypowerful technique for the study of the denaturation process of proteins. However, probingthe secondary structure is a difficult challenge and rarely yields quantitative values. The main purpose of this PhD project is to develop a technical implementation of far-UVcircular dichroism in a nanosecond T-jump experiment. Our CD/T-jump experiment allows usto follow quantitatively the change in the helical fraction of a poly(glutamic acid) peptideduring its thermal denaturation with 12 ns time resolution. pastel-00656201,version1-3Jan2012
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